Fertilisation is a multistep process that culminates in the fusion of sperm and egg, thus marking the beginning of life. The molecular mechanisms that regulate this singular event, particularly sperm–egg fusion, have remained mysterious for many decades. Through this course, we will learn about the biology behind fertilisation, the factors that can determine Fertilisation in the Laboratory and also understand poor and abnormal fertilisation and what to do when we face such situations. This course is intended for Embryologists, Scientists, IVF clinicians and even medical students wanting to understand the enigma that is fertilisation.